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KMID : 0545120000100050724
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 5 p.724 ~ p.727
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Degradation of Phenanthrene by Sphingomonas sp. 1-21 Isolated from Oil Contaminated Soil
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RYEOM, TAI-KYUNG
LEE, IL-GYU/SON, SEUNG-YEOL/AHN, TAE-YOUNG
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Abstract
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A phenanthrene-degrading bacterium, Strain 1-21, was isolated from oil-contaminated soil. This strain was a Gramnegative, aerobic, and rod-shaped bacterium, and exhibited a 99% sequence similarity of 16S rDNA to that of Sphingomonas subarctica. The major cellular fatty acid was a summed feature 7 (18:1 w7c, 18:1 w9t, 18:1 w12t), which is a characteristic of the Sphingomonas species. When 200 and 1,000ppm of phenanthrene was added as the sole carbon source, Strain 1-21 degraded 98% and 67% after 10 days of incubation, respectively. Futhermore, this strain was also able to utilize naphthalene and fluorene as sole carbon and energy sources.
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KEYWORD
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